Hotspot

rpl16

LSC · 81808-83133 · 1326 bp

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Pi 0.0144
MarkerSeek score 56.90
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1326
variable_sites
46
pi
0.0144
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ACACATCAATTCTCGAGCCC ACTTGGCTTTCCCTTCGATC 50.0 50.0 58.0 57.9 1634-1673 bp 1.0 83.0
rpl16_p2 CTGACCACCTCTGATCTTGC ACTTGGCTTTCCCTTCGATC 55.0 50.0 58.0 57.9 1747-1786 bp 1.0 82.8
rpl16_p3 GCATATCGACGATTGCTTGC ACTTGGCTTTCCCTTCGATC 50.0 50.0 58.1 57.9 1669-1708 bp 1.0 82.7
rpl16_p4 CAAAAGGGTCTGGGGTTGAA ACTTGGCTTTCCCTTCGATC 50.0 50.0 57.9 57.9 1595-1634 bp 1.0 82.5
rpl16_p5 TTCAAAAGGGTCTGGGGTTG ACTTGGCTTTCCCTTCGATC 50.0 50.0 57.9 57.9 1597-1636 bp 1.0 82.5