Hotspot

psaC-ndhE

SSC · 117929-118213 · 285 bp

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Pi 0.036
MarkerSeek score 67.80
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
285
variable_sites
19
pi
0.036
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 TACATCCGTCCCAGGGTATC GCGTTAATAGGTGCGATTGC 55.0 50.0 58.0 58.0 933-953 bp 1.0 84.2
psaC-ndhE_p2 CTGTTCTTGGAGCAGAAGCA GCGTTAATAGGTGCGATTGC 50.0 50.0 58.1 58.0 966-986 bp 1.0 83.2
psaC-ndhE_p3 AAATTACGGAGCCTGCGAAT GCGTTAATAGGTGCGATTGC 45.0 50.0 58.0 58.0 1255-1275 bp 1.0 83.1
psaC-ndhE_p4 AACCCGAACGCTCAAGAAAT GCGTTAATAGGTGCGATTGC 45.0 50.0 58.1 58.0 1031-1051 bp 1.0 83.1
psaC-ndhE_p5 CCTATTTGCCCGCTGTACAT GCGTTAATAGGTGCGATTGC 50.0 50.0 58.0 58.0 1571-1591 bp 1.0 82.6