Hotspot

trnL-UAG-ccsA

SSC · 114765-114868 · 104 bp

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Pi 0.0372
MarkerSeek score 64.10
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
104
variable_sites
9
pi
0.0372
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT TGCTCTAAAGTTGAAAATATCAT 50.0 26.1 58.0 52.2 196-198 bp 1.0 44.5
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG ATGCTCTAAAGTTGAAAATATCA 50.0 26.1 58.0 52.2 196-198 bp 1.0 44.5
trnL-UAG-ccsA_p3 TGAAATCGGTAGACACGCTG TGCTCTAAAGTTGAAAATATCAT 50.0 26.1 58.0 52.2 195-197 bp 1.0 44.5
trnL-UAG-ccsA_p4 GTGAAATCGGTAGACACGCT ATGCTCTAAAGTTGAAAATATCA 50.0 26.1 58.0 52.2 197-199 bp 1.0 44.4
trnL-UAG-ccsA_p5 TGAATTCGAACAAGCCGCTA TGCTCTAAAGTTGAAAATATCAT 45.0 26.1 57.9 52.2 218-220 bp 1.0 44.0