Pi
0.0163
MarkerSeek score
61.80
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AAAACCTGACCTGTTGACCC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1399-1533 bp | 1.0 | 86.6 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 741-879 bp | 1.0 | 85.7 |
| trnT-UGU-trnL-UAA_p3 | AAAACCTGACCTGTTGACCC | GGCTTTTTGGGGATAGAGGG | 50.0 | 55.0 | 57.9 | 57.9 | 1974-2108 bp | 1.0 | 85.6 |
| trnT-UGU-trnL-UAA_p4 | AAAACCTGACCTGTTGACCC | GGGCTTTTTGGGGATAGAGG | 50.0 | 55.0 | 57.9 | 57.9 | 1975-2109 bp | 1.0 | 85.6 |
| trnT-UGU-trnL-UAA_p5 | AAAACCTGACCTGTTGACCC | GCTTCCATTGAGTCTCTGCA | 50.0 | 50.0 | 57.9 | 57.9 | 1580-1714 bp | 1.0 | 85.5 |