Hotspot

rps4-trnT-UGU

LSC · 47108-47481 · 374 bp

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Pi 0.0259
MarkerSeek score 64.30
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
374
variable_sites
26
pi
0.0259
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 AAAACCTGACCTGTTGACCC CGAAGCAGAGCGCAATAATG 50.0 50.0 57.9 58.0 1047-1181 bp 1.0 87.1
rps4-trnT-UGU_p2 AATAGTCGGAGCCATACCCA CGAAGCAGAGCGCAATAATG 50.0 50.0 57.9 58.0 1111-1245 bp 1.0 86.7
rps4-trnT-UGU_p3 ATAGTCGGAGCCATACCCAA CGAAGCAGAGCGCAATAATG 50.0 50.0 57.9 58.0 1110-1244 bp 1.0 86.7
rps4-trnT-UGU_p4 TAGTCGGAGCCATACCCAAT CGAAGCAGAGCGCAATAATG 50.0 50.0 57.9 58.0 1109-1243 bp 1.0 86.7
rps4-trnT-UGU_p5 GTTTAGGTCTGGCGCGAATA CGAAGCAGAGCGCAATAATG 50.0 50.0 58.1 58.0 1407-1541 bp 1.0 86.5