Hotspot

petN-psbM

LSC · 29097-30170 · 1074 bp

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Pi 0.0148
MarkerSeek score 61.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1074
variable_sites
35
pi
0.0148
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGCTACCGCATTGTTCATTC 45.0 45.0 58.0 56.8 1233-1269 bp 1.0 74.7
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA TGCTACCGCATTGTTCATTCT 45.0 42.9 58.0 58.3 1233-1269 bp 1.0 74.0
petN-psbM_p3 AGTAAGTCTTGCTTGGGCTG TGCTACCGCATTGTTCATTC 50.0 45.0 57.8 56.8 1239-1275 bp 1.0 73.2
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG TGCTACCGCATTGTTCATTCT 50.0 42.9 57.8 58.3 1239-1275 bp 1.0 72.5
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA GCTACCGCATTGTTCATTCT 45.0 45.0 58.0 56.5 1232-1268 bp 1.0 72.3