Hotspot

trnK-UUU-rps16

LSC · 4130-4896 · 767 bp

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Pi 0.018
MarkerSeek score 64.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
767
variable_sites
29
pi
0.018
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.0 55.5 843-873 bp 1.0 66.0
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AAAGGCGGAGGTTTTTAAGT 55.0 40.0 57.9 55.5 860-890 bp 1.0 65.6
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AAAGGCGGAGGTTTTTAAGT 55.0 40.0 57.9 55.5 859-889 bp 1.0 65.6
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.2 55.5 799-829 bp 1.0 65.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.2 55.5 798-828 bp 1.0 65.1