primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	75	257	249-269	1.000000	82.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	75	257	299-319	1.000000	82.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	75	257	313-333	1.000000	82.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	75	257	312-332	1.000000	82.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	75	257	314-334	1.000000	82.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCCCTCGCTTCATCCAAATG	AAAGGCGGAGGTTTTTAAGT	20	20	50.0	40.0	58.0	55.5	0.0	0.0	2.588179	4130	4896	843-873	1.000000	66.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	AAAGGCGGAGGTTTTTAAGT	20	20	55.0	40.0	57.9	55.5	0.0	0.0	2.625779	4130	4896	860-890	1.000000	65.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGATGGTATGGACGAATCCC	AAAGGCGGAGGTTTTTAAGT	20	20	55.0	40.0	57.9	55.5	0.0	0.0	2.625779	4130	4896	859-889	1.000000	65.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAAGGCGGAGGTTTTTAAGT	20	20	50.0	40.0	58.2	55.5	20.3	0.0	2.713913	4130	4896	799-829	1.000000	65.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAAGGCGGAGGTTTTTAAGT	20	20	50.0	40.0	58.2	55.5	20.3	0.0	2.713913	4130	4896	798-828	1.000000	65.1
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7858	8254	517-518	1.000000	81.5
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7858	8254	518-519	1.000000	81.5
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	20	20	50.0	50.0	57.9	58.3	1.4	0.7	0.348988	7858	8254	519-520	1.000000	79.7
psbK-psbI_p4	psbK-psbI	4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	7858	8254	514-515	1.000000	79.5
psbK-psbI_p5	psbK-psbI	5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	7858	8254	515-516	1.000000	79.5
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9886	10072	266-304	1.000000	72.8
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9886	10072	269-307	1.000000	72.5
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9886	10072	272-310	1.000000	72.0
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9886	10072	275-313	1.000000	71.7
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9886	10072	270-308	1.000000	70.3
atpI-rps2_p1	atpI-rps2	1	AACCCGTTTAGTGCGTTGAT	TGTGAAGGGCGTTCTAGCTA	20	20	45.0	50.0	58.1	58.4	0.0	19.4	0.560535	15317	15600	361-491	1.000000	82.6
atpI-rps2_p2	atpI-rps2	2	AACCCGTTTAGTGCGTTGAT	TTTGTGAAGGGCGTTCTAGC	20	20	45.0	50.0	58.1	58.5	0.0	19.4	0.593705	15317	15600	363-493	1.000000	82.3
atpI-rps2_p3	atpI-rps2	3	AACCCGTTTAGTGCGTTGAT	GCAATTTGTGAAGGGCGTTC	20	20	45.0	50.0	58.1	58.6	0.0	12.8	0.694741	15317	15600	367-497	1.000000	81.4
atpI-rps2_p4	atpI-rps2	4	ATGTTGGCCTACTTCCACAC	TGTGAAGGGCGTTCTAGCTA	20	20	50.0	50.0	57.8	58.4	18.3	19.4	0.647794	15317	15600	395-525	1.000000	81.3
atpI-rps2_p5	atpI-rps2	5	ATGTTGGCCTACTTCCACAC	TTTGTGAAGGGCGTTCTAGC	20	20	50.0	50.0	57.8	58.5	18.3	19.4	0.680964	15317	15600	397-527	1.000000	81.0
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACCGCATTGTTCATTC	20	20	45.0	45.0	58.0	56.8	0.0	0.0	1.208847	29097	30170	1233-1269	1.000000	74.7
petN-psbM_p2	petN-psbM	2	TCTTGCTTGGGCTGCTTTAA	TGCTACCGCATTGTTCATTCT	20	21	45.0	42.9	58.0	58.3	0.0	0.0	1.295667	29097	30170	1233-1269	1.000000	74.0
petN-psbM_p3	petN-psbM	3	AGTAAGTCTTGCTTGGGCTG	TGCTACCGCATTGTTCATTC	20	20	50.0	45.0	57.8	56.8	0.0	0.0	1.393931	29097	30170	1239-1275	1.000000	73.2
petN-psbM_p4	petN-psbM	4	AGTAAGTCTTGCTTGGGCTG	TGCTACCGCATTGTTCATTCT	20	21	50.0	42.9	57.8	58.3	0.0	0.0	1.480751	29097	30170	1239-1275	1.000000	72.5
petN-psbM_p5	petN-psbM	5	TCTTGCTTGGGCTGCTTTAA	GCTACCGCATTGTTCATTCT	20	20	45.0	45.0	58.0	56.5	0.0	0.0	1.503900	29097	30170	1232-1268	1.000000	72.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	ACCCAAGAAGAGCCCATTTG	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.077102	31178	32060	638-1130	1.000000	89.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	ACCCAAGAAGAGCCCATTTG	TGGTGATTCCAGAGACGACT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.146401	31178	32060	892-1388	1.000000	88.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AAACCGGAGTCAGAAGGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.017093	31178	32060	1260-1578	1.000000	88.1
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AAACCGGAGTCAGAAGGAGA	TGGTGATTCCAGAGACGACT	20	20	50.0	50.0	58.0	58.1	9.5	0.0	0.086391	31178	32060	1505-1836	1.000000	87.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AAACCGGAGTCAGAAGGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	58.0	58.2	9.5	0.0	0.162026	31178	32060	1257-1575	1.000000	87.0
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	AAAACCTGACCTGTTGACCC	CGAAGCAGAGCGCAATAATG	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.082006	47108	47481	1047-1181	1.000000	87.1
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	AATAGTCGGAGCCATACCCA	CGAAGCAGAGCGCAATAATG	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.111751	47108	47481	1111-1245	1.000000	86.7
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	ATAGTCGGAGCCATACCCAA	CGAAGCAGAGCGCAATAATG	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.111751	47108	47481	1110-1244	1.000000	86.7
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TAGTCGGAGCCATACCCAAT	CGAAGCAGAGCGCAATAATG	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.111751	47108	47481	1109-1243	1.000000	86.7
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GTTTAGGTCTGGCGCGAATA	CGAAGCAGAGCGCAATAATG	20	20	50.0	50.0	58.1	58.0	12.1	11.5	0.084074	47108	47481	1407-1541	1.000000	86.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AAAACCTGACCTGTTGACCC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.071989	47555	48327	1399-1533	1.000000	86.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47555	48327	741-879	1.000000	85.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAAACCTGACCTGTTGACCC	GGCTTTTTGGGGATAGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.140618	47555	48327	1974-2108	1.000000	85.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAAACCTGACCTGTTGACCC	GGGCTTTTTGGGGATAGAGG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.140618	47555	48327	1975-2109	1.000000	85.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAAACCTGACCTGTTGACCC	GCTTCCATTGAGTCTCTGCA	20	20	50.0	50.0	57.9	57.9	0.0	0.6	0.172372	47555	48327	1580-1714	1.000000	85.5
accD-psaI_p1	accD-psaI	1	AAGCCGTTCAAACAGGCATA	TTTTCGCGACCCCGTTATAG	20	20	45.0	50.0	58.1	58.1	21.8	9.1	0.170484	59409	59889	1438-1673	1.000000	85.0
accD-psaI_p2	accD-psaI	2	GATCCGGATACTTGGGATGC	TTTTCGCGACCCCGTTATAG	20	20	55.0	50.0	57.9	58.1	19.1	9.1	0.175375	59409	59889	1562-1797	1.000000	84.9
accD-psaI_p3	accD-psaI	3	AAGCCGTTCAAACAGGCATA	TGGGGCCCATCAGATCTAAA	20	20	45.0	50.0	58.1	58.1	21.8	24.8	0.189874	59409	59889	1129-1164	0.666667	70.0
accD-psaI_p4	accD-psaI	4	AAGCCGTTCAAACAGGCATA	TTTACCCCCATTACCCCCAT	20	20	45.0	50.0	58.1	58.0	21.8	0.0	0.113869	59409	59889	1304-1304	0.166667	45.8
accD-psaI_p5	accD-psaI	5	GATCCGGATACTTGGGATGC	TTTACCCCCATTACCCCCAT	20	20	55.0	50.0	57.9	58.0	19.1	0.0	0.118760	59409	59889	1428-1428	0.166667	45.7
rpl16_p1	rpl16	1	ACACATCAATTCTCGAGCCC	ACTTGGCTTTCCCTTCGATC	20	20	50.0	50.0	58.0	57.9	7.8	0.0	0.145181	81808	83133	1634-1673	1.000000	83.0
rpl16_p2	rpl16	2	CTGACCACCTCTGATCTTGC	ACTTGGCTTTCCCTTCGATC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.136698	81808	83133	1747-1786	1.000000	82.8
rpl16_p3	rpl16	3	GCATATCGACGATTGCTTGC	ACTTGGCTTTCCCTTCGATC	20	20	50.0	50.0	58.1	57.9	24.8	0.0	0.172758	81808	83133	1669-1708	1.000000	82.7
rpl16_p4	rpl16	4	CAAAAGGGTCTGGGGTTGAA	ACTTGGCTTTCCCTTCGATC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.195997	81808	83133	1595-1634	1.000000	82.5
rpl16_p5	rpl16	5	TTCAAAAGGGTCTGGGGTTG	ACTTGGCTTTCCCTTCGATC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.195997	81808	83133	1597-1636	1.000000	82.5
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGACGGAAAAAGAAG	AGGCTTTTGTCGATTGGGTT	20	20	50.0	45.0	58.0	58.0	20.5	0.0	0.037038	113066	113593	1323-2047	1.000000	87.5
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGACGGAAAAAGAAGAA	AGGCTTTTGTCGATTGGGTT	20	20	45.0	45.0	58.0	58.0	20.5	0.0	0.048327	113066	113593	1321-2045	1.000000	87.4
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGACGGAAAAAGAAGA	AGGCTTTTGTCGATTGGGTT	20	20	45.0	45.0	58.0	58.0	20.5	0.0	0.048327	113066	113593	1322-2046	1.000000	87.4
ndhF-rpl32_p4	ndhF-rpl32	4	CCGTGTGAACCAAAACCCTA	AGGCTTTTGTCGATTGGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	113066	113593	1729-2453	1.000000	86.4
ndhF-rpl32_p5	ndhF-rpl32	5	CCGTGTGAACCAAAACCCTA	GGCTTTTGTCGATTGGGTTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094482	113066	113593	1728-2452	1.000000	86.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ATTTACCGGATCCTACCCCC	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.3	58.0	9.5	0.0	0.269357	113759	114684	1613-2340	1.000000	85.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ATTTACCGGATCCTACCCCC	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.3	58.0	9.5	0.0	0.270727	113759	114684	1614-2341	1.000000	85.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	113759	114684	984-1061	0.833333	80.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	113759	114684	985-1062	0.833333	80.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	113759	114684	994-1071	0.833333	79.9
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	TGCTCTAAAGTTGAAAATATCAT	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.840083	114765	114868	196-198	1.000000	44.5
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	ATGCTCTAAAGTTGAAAATATCA	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.841454	114765	114868	196-198	1.000000	44.5
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	TGAAATCGGTAGACACGCTG	TGCTCTAAAGTTGAAAATATCAT	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.841454	114765	114868	195-197	1.000000	44.5
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	GTGAAATCGGTAGACACGCT	ATGCTCTAAAGTTGAAAATATCA	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.840083	114765	114868	197-199	1.000000	44.4
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TGAATTCGAACAAGCCGCTA	TGCTCTAAAGTTGAAAATATCAT	20	23	45.0	26.1	57.9	52.2	0.0	0.0	8.919754	114765	114868	218-220	1.000000	44.0
psaC-ndhE_p1	psaC-ndhE	1	TACATCCGTCCCAGGGTATC	GCGTTAATAGGTGCGATTGC	20	20	55.0	50.0	58.0	58.0	5.0	0.0	0.024087	117929	118213	933-953	1.000000	84.2
psaC-ndhE_p2	psaC-ndhE	2	CTGTTCTTGGAGCAGAAGCA	GCGTTAATAGGTGCGATTGC	20	20	50.0	50.0	58.1	58.0	5.7	0.0	0.130502	117929	118213	966-986	1.000000	83.2
psaC-ndhE_p3	psaC-ndhE	3	AAATTACGGAGCCTGCGAAT	GCGTTAATAGGTGCGATTGC	20	20	45.0	50.0	58.0	58.0	14.0	0.0	0.057104	117929	118213	1255-1275	1.000000	83.1
psaC-ndhE_p4	psaC-ndhE	4	AACCCGAACGCTCAAGAAAT	GCGTTAATAGGTGCGATTGC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.126508	117929	118213	1031-1051	1.000000	83.1
psaC-ndhE_p5	psaC-ndhE	5	CCTATTTGCCCGCTGTACAT	GCGTTAATAGGTGCGATTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051399	117929	118213	1571-1591	1.000000	82.6
ndhE_p1	ndhE	1	TACATCCGTCCCAGGGTATC	GCGTTAATAGGTGCGATTGC	20	20	55.0	50.0	58.0	58.0	5.0	0.0	0.024087	118214	118519	933-953	1.000000	84.2
ndhE_p2	ndhE	2	TACATCCGTCCCAGGGTATC	TTTCCTCTTTGGACCGTTGG	20	20	55.0	50.0	58.0	58.0	5.0	0.0	0.029711	118214	118519	1161-1181	1.000000	83.6
ndhE_p3	ndhE	3	AAATTACGGAGCCTGCGAAT	GCGTTAATAGGTGCGATTGC	20	20	45.0	50.0	58.0	58.0	14.0	0.0	0.057104	118214	118519	1255-1275	1.000000	83.1
ndhE_p4	ndhE	4	TACATCCGTCCCAGGGTATC	TCTTTCTGGGATCGGGTCTT	20	20	55.0	50.0	58.0	58.0	5.0	0.0	0.052749	118214	118519	1391-1411	1.000000	82.9
ndhE_p5	ndhE	5	AAATTACGGAGCCTGCGAAT	TTTCCTCTTTGGACCGTTGG	20	20	45.0	50.0	58.0	58.0	14.0	0.0	0.062727	118214	118519	1483-1503	1.000000	82.7
ycf1_p1	ycf1	1	AGAACTCCGAGGTGTCGTAT	TCCGGCCAAACTAACCAAAA	20	20	50.0	45.0	57.9	57.9	0.0	2.2	0.194607	126668	126787	720-747	1.000000	82.0
ycf1_p2	ycf1	2	AGAACTCCGAGGTGTCGTAT	ACGCAAACGAACCTTACCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.168105	126668	126787	936-963	0.833333	75.2
ycf1_p3	ycf1	3	AGAACTCCGAGGTGTCGTAT	AAAGATGTACGAATCCGGCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.176308	126668	126787	733-733	0.166667	45.3
ycf1_p4	ycf1	4	AGAACTCCGAGGTGTCGTAT	CCTTTGGTTCTGTTCACCCA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.191672	126668	126787	920-920	0.166667	45.1
ycf1_p5	ycf1	5	AGAACTCCGAGGTGTCGTAT	ACCTTTGGTTCTGTTCACCC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.193138	126668	126787	921-921	0.166667	45.1