Hotspot

ycf1

SSC · 120747-126173 · 5427 bp

Back to Sisyrinchium

Pi 0.0102

MarkerSeek score 54.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5427
variable_sites: 55
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATCACATAATCGTGCCTCT	TCCGCTTTTTCAGAAATGAT	42.9	35.0	56.9	53.5	212-221 bp	1.0	45.2
ycf1_p2	TCATCACATAATCGTGCCTCT	TCCGCTTTTTCAGAAATGATAGA	42.9	34.8	56.9	56.3	212-221 bp	1.0	45.2
ycf1_p3	TCATCACATAATCGTGCCTCT	CGCTTTTTCAGAAATGATAGAACG	42.9	37.5	56.9	57.2	210-219 bp	1.0	45.2
ycf1_p4	TCATCACATAATCGTGCCTCTT	TCCGCTTTTTCAGAAATGAT	40.9	35.0	57.6	53.5	212-221 bp	1.0	45.2
ycf1_p5	TCATCATCACATAATCGTGCCT	TCCGCTTTTTCAGAAATGAT	40.9	35.0	57.7	53.5	215-224 bp	1.0	45.1