Hotspot

petD

LSC · 73545-74752 · 1208 bp

Back to Sisyrinchium

Pi 0.00431

MarkerSeek score 45.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1208
variable_sites: 5
pi: 0.00431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	CAGTTCATCGATGGTCAGCA	AGAGAGATCGCGGATATCGT	50.0	50.0	58.0	57.9	2563-2571 bp	1.0	79.6
petD_p2	CAGTTCATCGATGGTCAGCA	AGGAAGAGGGCATTCGTTTC	50.0	50.0	58.0	57.9	2320-2328 bp	1.0	79.6
petD_p3	CAGTTCATCGATGGTCAGCA	GAGGAAGAGGGCATTCGTTT	50.0	50.0	58.0	57.9	2321-2329 bp	1.0	79.6
petD_p4	ATTCCTTACCTCGGGACCAA	AGAGAGATCGCGGATATCGT	50.0	50.0	58.0	57.9	2407-2415 bp	1.0	79.5
petD_p5	TCCTTACCTCGGGACCAAAT	AGAGAGATCGCGGATATCGT	50.0	50.0	58.0	57.9	2405-2413 bp	1.0	79.5