Hotspot

rpl32-trnL-UAG

SSC · 110305-111130 · 826 bp

Back to Sisyrinchium

Pi 0.00852
MarkerSeek score 64.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
826
variable_sites
7
pi
0.00852
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCTAAATCGGTTTCCACAGG AGCGTGTCTACCGATTTCAC 50.0 50.0 56.8 58.0 927-984 bp 1.0 71.2
rpl32-trnL-UAG_p2 GCTAAATCGGTTTCCACAGG CAGCGTGTCTACCGATTTCA 50.0 50.0 56.8 58.0 928-985 bp 1.0 71.2
rpl32-trnL-UAG_p3 AGCTAAATCGGTTTCCACAGG AGCGTGTCTACCGATTTCAC 47.6 50.0 58.3 58.0 928-985 bp 1.0 70.5
rpl32-trnL-UAG_p4 AGCTAAATCGGTTTCCACAGG CAGCGTGTCTACCGATTTCA 47.6 50.0 58.3 58.0 929-986 bp 1.0 70.5
rpl32-trnL-UAG_p5 GCTAAATCGGTTTCCACAGGA AGCGTGTCTACCGATTTCAC 47.6 50.0 58.3 58.0 927-984 bp 1.0 70.5