Hotspot

rpl23-trnI-CAU

IRb · 83166-83330 · 165 bp

Back to Sisyrinchium

Pi 0.0125

MarkerSeek score 62.60

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: IRb
length_bp: 165
variable_sites: 2
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	TGATTGTTCCCCAATAACCGA	GAATGGTTAAAGCGCCCAAC	42.9	50.0	57.3	58.0	284-289 bp	1.0	68.1
rpl23-trnI-CAU_p2	TGATTGTTCCCCAATAACCGA	CGATGAGCCCTGTTAAGCAT	42.9	50.0	57.3	58.0	312-317 bp	1.0	67.7
rpl23-trnI-CAU_p3	TGATTGTTCCCCAATAACCGA	AGCCCTGTTAAGCATCCATG	42.9	50.0	57.3	57.9	307-312 bp	1.0	67.5
rpl23-trnI-CAU_p4	TGATTGTTCCCCAATAACCGA	CCTGTTAAGCATCCATGGCT	42.9	50.0	57.3	57.9	304-309 bp	1.0	67.5
rpl23-trnI-CAU_p5	TGATTGTTCCCCAATAACCGA	TTCACGATGTACGATGAGCC	42.9	50.0	57.3	57.8	323-328 bp	1.0	66.3