Hotspot

rps3-rpl22

LSC · 80353-80423 · 71 bp

Back to Sisyrinchium

Pi 0.0588

MarkerSeek score 78.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 4
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GGGCGAACCAAAAGGAATGA	CGGAGGTCCCTTTGTGAAAA	50.0	50.0	58.7	58.0	248-256 bp	1.0	77.6
rps3-rpl22_p2	GGGCGAACCAAAAGGAATGA	CGAGGGCGTAGTTTTCCAAT	50.0	50.0	58.7	58.3	211-219 bp	1.0	76.4
rps3-rpl22_p3	CCAAGTCTGAAACCAAGTGGA	CGGAGGTCCCTTTGTGAAAA	47.6	50.0	58.1	58.0	213-221 bp	1.0	75.7
rps3-rpl22_p4	GGCGAACCAAAAGGAATGATG	CGGAGGTCCCTTTGTGAAAA	47.6	50.0	58.1	58.0	247-255 bp	1.0	74.7
rps3-rpl22_p5	CCAAGTCTGAAACCAAGTGGA	CGAGGGCGTAGTTTTCCAAT	47.6	50.0	58.1	58.3	176-184 bp	1.0	74.7