Hotspot

psaI-ycf4

LSC · 57040-57386 · 347 bp

Back to Sisyrinchium

Pi 0.0122

MarkerSeek score 62.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 4
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	ATGTTCTGATCGCCAGTTCA	45.0	45.0	58.0	57.2	429-435 bp	1.0	78.1
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	TGTTCTGATCGCCAGTTCAT	45.0	45.0	58.0	57.2	428-434 bp	1.0	78.1
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	ATGTTCTGATCGCCAGTTCA	45.0	45.0	57.9	57.2	423-429 bp	1.0	77.5
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	TGTTCTGATCGCCAGTTCAT	45.0	45.0	57.9	57.2	422-428 bp	1.0	77.5
psaI-ycf4_p5	GGCCTAGTATTTCCGGCAAT	TGTTCTGATCGCCAGTTCAT	50.0	45.0	57.7	57.2	436-442 bp	1.0	76.1