Hotspot

trnF-GAA-ndhJ

LSC · 46616-47239 · 624 bp

Back to Sisyrinchium

Pi 0.092

MarkerSeek score 89.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 56
pi: 0.092
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 89.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	TGTCACCAGTTCAAACCTGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.9	57.9	729-783 bp	1.0	86.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	734-788 bp	1.0	86.0
trnF-GAA-ndhJ_p3	TGTCACCAGTTCAAACCTGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	57.9	58.2	719-773 bp	1.0	85.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	724-778 bp	1.0	85.5
trnF-GAA-ndhJ_p5	TAGGATAATGCGCGGTAAGC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.9	57.9	794-848 bp	1.0	85.3