Hotspot

trnS-GCU-trnG-UCC

LSC · 8137-9135 · 999 bp

Back to Sisyrinchium

Pi 0.0147

MarkerSeek score 65.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 14
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCAACGGATTAGCAATCTGC	AACCGAAAGACCCCTTAACT	50.0	45.0	57.9	56.4	1089-1118 bp	1.0	70.5
trnS-GCU-trnG-UCC_p2	AATCTGCCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	1076-1105 bp	1.0	69.4
trnS-GCU-trnG-UCC_p3	CAATCTGCCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	1077-1106 bp	1.0	69.4
trnS-GCU-trnG-UCC_p4	CGGAGAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	1130-1159 bp	1.0	68.7
trnS-GCU-trnG-UCC_p5	GCAACGGATTAGCAATCTGC	AAACCGAAAGACCCCTTAACT	50.0	42.9	57.9	57.1	1090-1119 bp	1.0	68.6