Hotspot

ycf1

SSC · 123100-128388 · 5289 bp

Back to Sisymbrium

Pi 0.0162

MarkerSeek score 46.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5289
variable_sites: 235
pi: 0.0162
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTTACGCCTTTGGGAACG	CCCCCAACCTTTCCACTTTT	50.0	50.0	58.0	58.2	877 bp	1.0	82.6
ycf1_p2	CCAGTCCCCAAACCGTTAAA	CCCCCAACCTTTCCACTTTT	50.0	50.0	58.0	58.2	1467 bp	1.0	82.5
ycf1_p3	CCAGTCCCCAAACCGTTAAA	TCCATTTTTGCATGGGCTCT	50.0	45.0	58.0	58.1	1214 bp	0.166667	46.1
ycf1_p4	GTTTTACGCCTTTGGGAACG	TCCATTTTTGCATGGGCTCT	50.0	45.0	58.0	58.1	624 bp	0.166667	45.9
ycf1_p5	AGTCGAGCCTTCAGAGATGA	TCCATTTTTGCATGGGCTCT	50.0	45.0	57.9	58.1	1158 bp	0.166667	45.1