Hotspot

trnN-AAC-trnR-CGU

IRa · 128781-129397 · 617 bp

Back to Sisymbrium

Pi 0.00844

MarkerSeek score 62.30

Primer available yes

Label trnN-AAC-trnR-CGU

Feature Metadata

label_name: trnN-AAC-trnR-CGU
feature_type: igs
region: IRa
length_bp: 617
variable_sites: 14
pi: 0.00844
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-AAC-trnR-CGU_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	745-773 bp	1.0	81.5
trnN-AAC-trnR-CGU_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	782-810 bp	1.0	80.8
trnN-AAC-trnR-CGU_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	786-814 bp	1.0	80.6
trnN-AAC-trnR-CGU_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	696-724 bp	1.0	80.5
trnN-AAC-trnR-CGU_p5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	823-851 bp	1.0	79.9