Hotspot

ndhE-ndhG

SSC · 117501-117596 · 96 bp

Back to Sisymbrium

Pi 0.0438

MarkerSeek score 67.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 96
variable_sites: 11
pi: 0.0438
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GGCACTCAAAACAAGTACATGT	TAGGTGCAATTTCTGTGGCT	40.9	45.0	57.4	57.4	158-243 bp	1.0	58.6
ndhE-ndhG_p2	GGCACTCAAAACAAGTACATGT	AGGTGCAATTTCTGTGGCTC	40.9	50.0	57.4	58.7	157-242 bp	1.0	57.4
ndhE-ndhG_p3	AGGCACTCAAAACAAGTACATG	TAGGTGCAATTTCTGTGGCT	40.9	45.0	57.2	57.4	159-244 bp	1.0	56.5
ndhE-ndhG_p4	AGGCACTCAAAACAAGTACA	TAGGTGCAATTTCTGTGGCT	40.0	45.0	55.1	57.4	159-244 bp	1.0	56.1
ndhE-ndhG_p5	AGGCACTCAAAACAAGTACATG	AGGTGCAATTTCTGTGGCTC	40.9	50.0	57.2	58.7	158-243 bp	1.0	55.2