Hotspot

trnL-CUA-ccsA

SSC · 113736-113847 · 112 bp

Back to Sisymbrium

Pi 0.0274

MarkerSeek score 61.00

Primer available yes

Label trnL-CUA-ccsA

Feature Metadata

label_name: trnL-CUA-ccsA
feature_type: igs
region: SSC
length_bp: 112
variable_sites: 8
pi: 0.0274
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CUA-ccsA_p1	GTTTTGAACAAGTTGCCGCT	AGTACAATTGAAACGACCGA	45.0	40.0	58.1	55.1	271-276 bp	1.0	58.1
trnL-CUA-ccsA_p2	AAGTTGCCGCTATGGTGAAA	AGTACAATTGAAACGACCGA	45.0	40.0	58.1	55.1	262-267 bp	1.0	58.1
trnL-CUA-ccsA_p3	TAGACACGCTGCTCTTAGGA	AGTACAATTGAAACGACCGA	50.0	40.0	57.9	55.1	238-243 bp	1.0	58.1
trnL-CUA-ccsA_p4	AGTTGCCGCTATGGTGAAAT	AGTACAATTGAAACGACCGA	45.0	40.0	57.9	55.1	261-266 bp	1.0	57.8
trnL-CUA-ccsA_p5	AAATTGGTAGACACGCTGCT	AGTACAATTGAAACGACCGA	45.0	40.0	57.8	55.1	245-250 bp	1.0	57.5