Hotspot

ndhF-rpl32

SSC · 112005-112857 · 853 bp

Back to Sisymbrium

Pi 0.0258
MarkerSeek score 59.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
853
variable_sites
50
pi
0.0258
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.89
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCAATAGGTCGTGTGAACCA GGAAAGCGATTTCCCTAAGGA 50.0 47.6 58.2 57.7 1402-1482 bp 1.0 76.0
ndhF-rpl32_p2 TGCAATAGGTCGTGTGAACC GGAAAGCGATTTCCCTAAGGA 50.0 47.6 58.2 57.7 1403-1483 bp 1.0 76.0
ndhF-rpl32_p3 GCAATAGGTCGTGTGAACCA TCCTCTTAACGGGGAACTACA 50.0 47.6 58.2 57.8 1693 bp 0.166667 35.7
ndhF-rpl32_p4 TGCAATAGGTCGTGTGAACC TCCTCTTAACGGGGAACTACA 50.0 47.6 58.2 57.8 1694 bp 0.166667 35.7
ndhF-rpl32_p5 GGTCGTGTGAACCAAAAACC TCCTCTTAACGGGGAACTACA 50.0 47.6 57.8 57.8 1687 bp 0.166667 35.7