Hotspot

rpl36-rps8

LSC · 78962-79439 · 478 bp

Back to Sisymbrium

Pi 0.0307

MarkerSeek score 64.10

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 30
pi: 0.0307
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.7	58.4	476-538 bp	1.0	83.3
rpl36-rps8_p2	TTTTACGAACGGAAGCCCTT	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.7	57.6	475-537 bp	1.0	83.2
rpl36-rps8_p3	ACGAACGGAAGCCCTTATTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.5	58.4	472-534 bp	1.0	81.6
rpl36-rps8_p4	ATTTTACGAACGGAAGCCCT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.5	58.4	477-539 bp	1.0	81.5
rpl36-rps8_p5	ACGAACGGAAGCCCTTATTT	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.5	57.6	471-533 bp	1.0	81.5