Hotspot

psbE-petL

LSC · 63750-64840 · 1091 bp

Back to Sisymbrium

Pi 0.0254

MarkerSeek score 63.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 69
pi: 0.0254
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCTGT	CGAGCGCTCTTAGTTCAGTT	45.0	50.0	58.0	58.0	1913 bp	0.166667	46.5
psbE-petL_p2	AAGGATCAAAACGGCCTGTT	CGAGCGCTCTTAGTTCAGTT	45.0	50.0	58.0	58.0	1912 bp	0.166667	46.5
psbE-petL_p3	TCAGCCATTTATCCCGCTTC	CGAGCGCTCTTAGTTCAGTT	50.0	50.0	58.0	58.0	2365 bp	0.166667	46.3
psbE-petL_p4	CGGTAGGTACAGCTAGTCCA	CGAGCGCTCTTAGTTCAGTT	55.0	50.0	58.0	58.0	2029 bp	0.166667	46.2
psbE-petL_p5	ACGGTAGGTACAGCTAGTCC	CGAGCGCTCTTAGTTCAGTT	55.0	50.0	58.0	58.0	2030 bp	0.166667	46.1