Hotspot

petA-psbJ

LSC · 61991-62964 · 974 bp

Back to Sisymbrium

Pi 0.022
MarkerSeek score 62.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
974
variable_sites
56
pi
0.022
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTTGAGAAGGTTCA GCTGGTATTCCTGTGATCGG 36.4 55.0 55.8 58.1 1088-1128 bp 1.0 51.4
petA-psbJ_p2 AAGAAACAGTTTGAGAAGGTTCA GCTGGTATTCCTGTGATCGG 34.8 55.0 56.5 58.1 1089-1129 bp 1.0 49.1
petA-psbJ_p3 AGAAACAGTTTGAGAAGGTTCAA GCTGGTATTCCTGTGATCGG 34.8 55.0 56.5 58.1 1088-1128 bp 1.0 49.1
petA-psbJ_p4 TGAGAAGGTTCAATTATCCGA GCTGGTATTCCTGTGATCGG 38.1 55.0 54.4 58.1 1078-1118 bp 1.0 48.1
petA-psbJ_p5 GAAACAGTTTGAGAAGGTTCA GCTGGTATTCCTGTGATCGG 38.1 55.0 54.3 58.1 1087-1127 bp 1.0 47.2