Hotspot

trnD-GAC-trnY-UAC

LSC · 29780-30218 · 439 bp

Back to Sisymbrium

Pi 0.0243

MarkerSeek score 58.30

Primer available yes

Label trnD-GAC-trnY-UAC

Feature Metadata

label_name: trnD-GAC-trnY-UAC
feature_type: igs
region: LSC
length_bp: 439
variable_sites: 26
pi: 0.0243
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GAC-trnY-UAC_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	509-533 bp	1.0	78.2
trnD-GAC-trnY-UAC_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	508-532 bp	1.0	78.2
trnD-GAC-trnY-UAC_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	530-554 bp	1.0	77.2
trnD-GAC-trnY-UAC_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	529-553 bp	1.0	77.0
trnD-GAC-trnY-UAC_p5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	525-549 bp	1.0	75.9