Hotspot

psbK-psbI

LSC · 7178-7469 · 292 bp

Back to Sisymbrium

Pi 0.0331

MarkerSeek score 61.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 292
variable_sites: 23
pi: 0.0331
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCAGCTGTCAGTTTTCG	ACGGTGTATACAAAAAGTTTGAGA	50.0	33.3	58.0	56.8	347-354 bp	1.0	47.1
psbK-psbI_p2	CAAGCAGCTGTCAGTTTTCG	ACGGTGTATACAAAAAGTTTGAGAG	50.0	36.0	58.0	57.8	347-354 bp	1.0	47.1
psbK-psbI_p3	CAAGCAGCTGTCAGTTTTCG	CGGTGTATACAAAAAGTTTGAGAGT	50.0	36.0	58.0	57.8	346-353 bp	1.0	47.1
psbK-psbI_p4	AAGCAGCTGTCAGTTTTCGA	ACGGTGTATACAAAAAGTTTGAGA	45.0	33.3	58.0	56.8	346-353 bp	1.0	47.1
psbK-psbI_p5	AAGCAGCTGTCAGTTTTCGA	CGGTGTATACAAAAAGTTTGAGAGT	45.0	36.0	58.0	57.8	345-352 bp	1.0	47.1