Hotspot

rps16-trnQ-CAA

LSC · 6139-6585 · 447 bp

Back to Sisymbrium

Pi 0.0267

MarkerSeek score 65.40

Primer available yes

Label rps16-trnQ-CAA

Feature Metadata

label_name: rps16-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 32
pi: 0.0267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	AAGGTGGCGGATCAAAACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	566-596 bp	1.0	83.5
rps16-trnQ-CAA_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	520-550 bp	1.0	83.4
rps16-trnQ-CAA_p3	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	530-560 bp	1.0	82.5
rps16-trnQ-CAA_p4	AAAGGTGGCGGATCAAAACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.3	58.1	567-597 bp	1.0	81.7
rps16-trnQ-CAA_p5	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	546-576 bp	1.0	80.9