Hotspot

rpl16

LSC · 81031-82550 · 1520 bp

Back to Sisymbriopsis

Pi 0.00395

MarkerSeek score 47.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1520
variable_sites: 9
pi: 0.00395
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	CATGGAGGGAATTGCACGTA	50.0	50.0	58.0	58.0	2371-2375 bp	1.0	80.0
rpl16_p2	GTCTCAATTCCCGTGGGATC	CATGGAGGGAATTGCACGTA	55.0	50.0	58.0	58.0	2630-2634 bp	1.0	79.8
rpl16_p3	ATCGGCGATTACTAGCTCCT	CATGGAGGGAATTGCACGTA	50.0	50.0	58.1	58.0	2402-2406 bp	1.0	79.5
rpl16_p4	ACACATCAATTCTCGAGCCC	TGCACGTGTTGAATGGATCA	50.0	45.0	58.0	58.1	2020-2024 bp	1.0	79.5
rpl16_p5	GTCTCAATTCCCGTGGGATC	TGCACGTGTTGAATGGATCA	55.0	45.0	58.0	58.1	2279-2283 bp	1.0	79.3