Hotspot

ndhG-ndhI

SSC · 118983-119307 · 325 bp

Back to Sisymbriopsis

Pi 0.00821

MarkerSeek score 58.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 325
variable_sites: 4
pi: 0.00821
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATACCACTCCCAGACCTCC	TCAAATAGCTTTGGGTCGTT	55.0	40.0	58.1	55.3	499 bp	1.0	57.8
ndhG-ndhI_p2	TACCACTCCCAGACCTCCTA	TCAAATAGCTTTGGGTCGTT	55.0	40.0	58.3	55.3	497 bp	1.0	56.3
ndhG-ndhI_p3	AATACCACTCCCAGACCTCC	TCAAATAGCTTTGGGTCGTTT	55.0	38.1	58.1	56.1	499 bp	1.0	56.3
ndhG-ndhI_p4	AATACCACTCCCAGACCTCC	ATCAAATAGCTTTGGGTCGT	55.0	40.0	58.1	55.0	500 bp	1.0	55.9
ndhG-ndhI_p5	TACCACTCCCAGACCTCCTA	TCAAATAGCTTTGGGTCGTTT	55.0	38.1	58.3	56.1	497 bp	1.0	54.7