Hotspot

ccsA-ndhD

SSC · 115605-115819 · 215 bp

Back to Sisymbriopsis

Pi 0.0279

MarkerSeek score 69.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 215
variable_sites: 9
pi: 0.0279
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCTGCAATTGTGGCTTCT	AGCAAGTGACAAGGTCGAAT	40.0	45.0	55.9	57.4	374-377 bp	1.0	60.7
ccsA-ndhD_p2	TGGATATGCTATTTTGGCGTCA	AGCAAGTGACAAGGTCGAAT	40.9	45.0	58.2	57.4	336-339 bp	1.0	60.2
ccsA-ndhD_p3	TGCTATTTTGGCGTCAATCT	AGCAAGTGACAAGGTCGAAT	40.0	45.0	55.7	57.4	330-333 bp	1.0	59.6
ccsA-ndhD_p4	AATTCTGCAATTGTGGCTTCT	AGCAAGTGACAAGGTCGAAT	38.1	45.0	56.7	57.4	375-378 bp	1.0	58.9
ccsA-ndhD_p5	ATTCTGCAATTGTGGCTTCT	GCAAGTGACAAGGTCGAATC	40.0	50.0	55.9	57.1	373-376 bp	1.0	58.1