Hotspot

rpl32-trnL-CUA

SSC · 113756-114421 · 666 bp

Back to Sisymbriopsis

Pi 0.00602

MarkerSeek score 62.30

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 666
variable_sites: 6
pi: 0.00602
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	800-811 bp	1.0	65.6
rpl32-trnL-CUA_p2	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	793-804 bp	1.0	65.1
rpl32-trnL-CUA_p3	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	799-810 bp	1.0	65.1
rpl32-trnL-CUA_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	798-809 bp	1.0	65.1
rpl32-trnL-CUA_p5	GGAAGGGATATTGGACATCGT	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.2	58.3	794-805 bp	1.0	64.4