Hotspot

ndhF-rpl32

SSC · 112778-113596 · 819 bp

Back to Sisymbriopsis

Pi 0.00575
MarkerSeek score 63.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
819
variable_sites
7
pi
0.00575
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGGA ACGATGTCCAATATCCCTTCC 47.6 47.6 57.6 57.2 951-956 bp 1.0 55.6
ndhF-rpl32_p2 TTGTTGCTGTCGGAAAAAGT ACGATGTCCAATATCCCTTCC 40.0 47.6 56.4 57.2 999-1004 bp 1.0 54.2
ndhF-rpl32_p3 TGGAAGTGGAATGAAAGGGA ACGATGTCCAATATCCCTTCC 45.0 47.6 56.3 57.2 950-955 bp 1.0 53.8
ndhF-rpl32_p4 CTGGAAGTGGAATGAAAGGGA CGATGTCCAATATCCCTTCCT 47.6 47.6 57.6 56.9 950-955 bp 1.0 53.3
ndhF-rpl32_p5 CTGGAAGTGGAATGAAAGGGA AACGATGTCCAATATCCCTTCC 47.6 45.5 57.6 57.9 952-957 bp 1.0 53.0