Hotspot

rpl20-rps12

LSC · 68822-69581 · 760 bp

Back to Sisymbriopsis

Pi 0.00175

MarkerSeek score 59.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 2
pi: 0.00175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	873 bp	1.0	78.3
rpl20-rps12_p2	GAAGTTTTGTTCTGCGCCTC	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	891 bp	1.0	78.3
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	878 bp	1.0	77.7
rpl20-rps12_p4	GAAGTTTTGTTCTGCGCCTC	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	896 bp	1.0	77.7
rpl20-rps12_p5	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	817 bp	1.0	77.6