Hotspot

accD-psaI

LSC · 58553-59220 · 668 bp

Back to Sisymbriopsis

Pi 0.00299

MarkerSeek score 60.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 3
pi: 0.00299
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACGTAAGGGCTTGTTGGATG	AAAGAAGCCATTGCAAGTGC	50.0	45.0	58.2	57.8	851-863 bp	1.0	77.6
accD-psaI_p2	TGGATGCAATTGTACCACGT	AAAGAAGCCATTGCAAGTGC	45.0	45.0	57.8	57.8	837-849 bp	1.0	77.5
accD-psaI_p3	ACGTAAGGGCTTGTTGGATG	CCGGAAAGACTAGGCCTACT	50.0	55.0	58.2	58.2	832-844 bp	1.0	77.1
accD-psaI_p4	TGGATGCAATTGTACCACGT	CCGGAAAGACTAGGCCTACT	45.0	55.0	57.8	58.2	818-830 bp	1.0	77.0
accD-psaI_p5	GGGCTTGTTGGATGCAATTG	AAAGAAGCCATTGCAAGTGC	50.0	45.0	58.3	57.8	845-857 bp	1.0	76.8