Hotspot

ndhC-trnV-GUA

LSC · 50355-51241 · 887 bp

Back to Sisymbriopsis

Pi 0.00455
MarkerSeek score 49.90
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
887
variable_sites
6
pi
0.00455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TGCTAAAACAGGAATAGCACTTG GTTCGAGTCCGTATAGCCCT 39.1 55.0 57.4 58.7 973-980 bp 1.0 46.6
ndhC-trnV-GUA_p2 ACTCCGGAAATTAGAAATGCT GTTCGAGTCCGTATAGCCCT 38.1 55.0 55.3 58.7 990-997 bp 1.0 45.8
ndhC-trnV-GUA_p3 TGCTAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 38.1 55.0 55.2 58.7 973-980 bp 1.0 45.3
ndhC-trnV-GUA_p4 GCTAAAACAGGAATAGCACTTGA GTTCGAGTCCGTATAGCCCT 39.1 55.0 57.1 58.7 972-979 bp 1.0 44.6
ndhC-trnV-GUA_p5 TGCTAAAACAGGAATAGCACTTG TTCGAGTCCGTATAGCCCTA 39.1 50.0 57.4 57.1 972-979 bp 1.0 44.6