Hotspot

trnL-UUA-trnF-UUC

LSC · 47657-48221 · 565 bp

Back to Sisymbriopsis

Pi 0.00826

MarkerSeek score 54.60

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 7
pi: 0.00826
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	655-761 bp	1.0	76.6
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	652-758 bp	1.0	73.4
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	644-750 bp	1.0	72.7
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.5	57.4	631-737 bp	1.0	72.2
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TTCAGTCCTCTGCTCTACCA	50.0	50.0	57.5	57.4	632-738 bp	1.0	72.2