Hotspot

psaA-ycf3

LSC · 41799-42513 · 715 bp

Back to Sisymbriopsis

Pi 0.00373

MarkerSeek score 61.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 4
pi: 0.00373
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GCTATAACGCTTACTCCCGG	50.0	55.0	57.9	57.9	863-867 bp	1.0	79.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGCTATAACGCTTACTCCCG	50.0	55.0	57.9	57.9	864-868 bp	1.0	79.3
psaA-ycf3_p3	AGAAATGACCGGGTTTAGCC	GCTATAACGCTTACTCCCGG	50.0	55.0	57.9	57.9	880-884 bp	1.0	79.2
psaA-ycf3_p4	AGAAATGACCGGGTTTAGCC	GGCTATAACGCTTACTCCCG	50.0	55.0	57.9	57.9	881-885 bp	1.0	79.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACCATCACGAGG	50.0	50.0	57.9	57.9	823-827 bp	1.0	78.9