Hotspot

trnE-GAA-trnT-ACC

LSC · 30526-31367 · 842 bp

Back to Sisymbriopsis

Pi 0.00598

MarkerSeek score 59.30

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 6
pi: 0.00598
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	716-983 bp	1.0	83.2
trnE-GAA-trnT-ACC_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	695-962 bp	1.0	82.8
trnE-GAA-trnT-ACC_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	692-959 bp	1.0	82.6
trnE-GAA-trnT-ACC_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	678-945 bp	1.0	82.4
trnE-GAA-trnT-ACC_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	657-924 bp	1.0	82.3