Hotspot

psbM-trnD-GAC

LSC · 28771-29793 · 1023 bp

Back to Sisymbriopsis

Pi 0.00522

MarkerSeek score 62.30

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 8
pi: 0.00522
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	TGAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.5	50.0	58.1	58.5	1136-1139 bp	1.0	59.7
psbM-trnD-GAC_p2	GAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	56.4	58.5	1135-1138 bp	1.0	56.4
psbM-trnD-GAC_p3	AGGAATGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1141-1144 bp	1.0	56.4
psbM-trnD-GAC_p4	TGAGAATGAAGAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.5	50.0	58.1	57.0	1137-1140 bp	1.0	55.6
psbM-trnD-GAC_p5	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	1134-1137 bp	1.0	54.3