Hotspot

ycf1

SSC · 128099-133648 · 5550 bp

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Pi 0.00433

MarkerSeek score 44.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5550
variable_sites: 24
pi: 0.00433
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAGTACATCCCGAGCTAGA	AAAGTGACGGAAAAGAGAGA	55.0	40.0	58.0	54.3	234 bp	1.0	51.6
ycf1_p2	CCAGTACATCCCGAGCTAGA	AAGTGACGGAAAAGAGAGAA	55.0	40.0	58.0	54.3	233 bp	1.0	51.6
ycf1_p3	TCCAGTACATCCCGAGCTAG	AAAGTGACGGAAAAGAGAGA	55.0	40.0	58.0	54.3	235 bp	1.0	51.6
ycf1_p4	TCCAGTACATCCCGAGCTAG	AAGTGACGGAAAAGAGAGAA	55.0	40.0	58.0	54.3	234 bp	1.0	51.6
ycf1_p5	CCAGTACATCCCGAGCTAGA	AAAGTGACGGAAAAGAGAGAA	55.0	38.1	58.0	55.1	234 bp	1.0	50.4