Hotspot

rpl32-trnL-TAG

SSC · 116974-118349 · 1376 bp

Back to Siraitia

Pi 0.00819
MarkerSeek score 65.80
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
1376
variable_sites
11
pi
0.00819
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 GGCAGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.4 58.0 1479-1490 bp 1.0 78.9
rpl32-trnL-TAG_p2 GGCAGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.4 58.0 1480-1491 bp 1.0 78.9
rpl32-trnL-TAG_p3 AGCGTTGAAAGCTTTTTCGC AGCGTGTCTACCGATTTCAC 45.0 50.0 58.5 58.0 1476-1487 bp 1.0 78.1
rpl32-trnL-TAG_p4 GCGTTGAAAGCTTTTTCGCT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.5 58.0 1475-1486 bp 1.0 78.1
rpl32-trnL-TAG_p5 GCGTTGAAAGCTTTTTCGCT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.5 58.0 1476-1487 bp 1.0 78.1