Hotspot

ndhF-rpl32

SSC · 116152-116811 · 660 bp

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Pi 0.00916

MarkerSeek score 61.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 660
variable_sites: 6
pi: 0.00916
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	CGCCCTTTGCTTTTCCAAAT	45.0	45.0	58.0	57.8	833-834 bp	1.0	79.3
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	CGCCCTTTGCTTTTCCAAAT	45.0	45.0	57.8	57.8	835-836 bp	1.0	78.1
ndhF-rpl32_p3	TGTTGCCGTCGGAAAAAGTA	AAAAAGCTTTCAACGCTGCC	45.0	45.0	58.0	58.4	856-857 bp	1.0	77.2
ndhF-rpl32_p4	TGTTGCCGTCGGAAAAAGTA	AGCGAAAAAGCTTTCAACGC	45.0	45.0	58.0	58.5	860-861 bp	1.0	76.4
ndhF-rpl32_p5	TGTTGCCGTCGGAAAAAGTA	GCGAAAAAGCTTTCAACGCT	45.0	45.0	58.0	58.5	859-860 bp	1.0	76.4