Hotspot

trnM-CAU-atpE

LSC · 55360-55533 · 174 bp

Back to Siraitia

Pi 0.0462

MarkerSeek score 74.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 8
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	CGATGTGATAGGGCCCATTT	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.7	58.0	342-343 bp	1.0	79.6
trnM-CAU-atpE_p2	CGATGTGATAGGGCCCATTT	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.7	58.1	334-335 bp	1.0	79.4
trnM-CAU-atpE_p3	TATCGATGTGATAGGGCCCA	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.7	58.0	345-346 bp	1.0	79.3
trnM-CAU-atpE_p4	ATCGATGTGATAGGGCCCAT	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.3	58.0	344-345 bp	1.0	79.1
trnM-CAU-atpE_p5	TATCGATGTGATAGGGCCCA	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.7	58.1	337-338 bp	1.0	79.1