Hotspot

ndhC-trnV

LSC · 53647-54436 · 790 bp

Back to Siraitia

Pi 0.0228

MarkerSeek score 58.00

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 18
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	AGAGAAGCGGCCATAAACTG	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1522-1539 bp	1.0	80.5
ndhC-trnV_p2	AGAGAAGCGGCCATAAACTG	ACAAGCACAAGGGCTATAGC	50.0	50.0	58.0	58.0	2004-2021 bp	1.0	80.2
ndhC-trnV_p3	AGAGAAGCGGCCATAAACTG	CGAATCGAGTGCCCCATTTA	50.0	50.0	58.0	58.1	1827-1844 bp	1.0	80.2
ndhC-trnV_p4	TGGTTCAGGCATTTGCTCAT	TGAATCAGAGCACATGGAGC	45.0	50.0	58.1	58.0	1704-1721 bp	1.0	80.1
ndhC-trnV_p5	TGGTTCAGGCATTTGCTCAT	ACAAGCACAAGGGCTATAGC	45.0	50.0	58.1	58.0	2186-2203 bp	1.0	79.8