Hotspot

ndhJ-ndhK

LSC · 52448-52560 · 113 bp

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Pi 0.0177

MarkerSeek score 62.70

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 2
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	AGAAGACAAATGACCCTGCA	TCAGAGATCATCCCTCCTGA	45.0	50.0	57.0	56.5	206-210 bp	1.0	61.4
ndhJ-ndhK_p2	GCCAAGAAGACAAATGACCC	TCAGAGATCATCCCTCCTGA	50.0	50.0	57.0	56.5	210-214 bp	1.0	60.9
ndhJ-ndhK_p3	CAGTCCATGTTTGACTAGCCA	TCAGAGATCATCCCTCCTGA	47.6	50.0	57.9	56.5	227-231 bp	1.0	60.5
ndhJ-ndhK_p4	ACCAGTCCATGTTTGACTAGC	TCAGAGATCATCCCTCCTGA	47.6	50.0	57.9	56.5	229-233 bp	1.0	60.5
ndhJ-ndhK_p5	AGAAGACAAATGACCCTGCA	TCAGAGATCATCCCTCCTGAA	45.0	47.6	57.0	57.3	206-210 bp	1.0	59.5