Hotspot

rpoB-trnC-GCA

LSC · 28432-29370 · 939 bp

Back to Siraitia

Pi 0.0064

MarkerSeek score 62.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 939
variable_sites: 6
pi: 0.0064
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1025-1239 bp	1.0	78.8
rpoB-trnC-GCA_p2	CTCTCATTTCCACCCCCAAG	TGGGGAAAAAGGGATTTGCA	55.0	45.0	57.9	57.9	1013-1227 bp	1.0	78.5
rpoB-trnC-GCA_p3	TCTCTCATTTCCACCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1026-1240 bp	1.0	77.9
rpoB-trnC-GCA_p4	TTCTCTCATTTCCACCCCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1027-1241 bp	1.0	77.9
rpoB-trnC-GCA_p5	TCTCTCATTTCCACCCCCAA	TGGGGAAAAAGGGATTTGCA	50.0	45.0	58.2	57.9	1014-1228 bp	1.0	77.7