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psbK-psbI

LSC · 8334-8724 · 391 bp

Back to Siraitia

Pi 0.0106

MarkerSeek score 63.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 4
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTGCCGTAAGCTTTCGATGA	TTACGTCCCGGGTCATTAGA	50.0	50.0	58.4	57.9	502-503 bp	1.0	78.5
psbK-psbI_p2	CTGCCGTAAGCTTTCGATGA	ACGTCCCGGGTCATTAGATA	50.0	50.0	58.4	57.6	500-501 bp	1.0	76.7
psbK-psbI_p3	CTGCCGTAAGCTTTCGATGA	TACGTCCCGGGTCATTAGAT	50.0	50.0	58.4	57.6	501-502 bp	1.0	76.7
psbK-psbI_p4	CTGCCGTAAGCTTTCGATGA	TTCTTCACGTCCCGGATTAC	50.0	50.0	58.4	57.3	518-519 bp	1.0	74.3
psbK-psbI_p5	CTGCCGTAAGCTTTCGATGA	CGTCCCGGGTCATTAGATAG	50.0	55.0	58.4	57.0	499-500 bp	1.0	71.6