Hotspot

rps15-ycf1

SSC · 121518-121942 · 425 bp

Back to Siphocranion

Pi 0.0223

MarkerSeek score 57.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 425
variable_sites: 9
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTCGTTGACGTTTTCCCAA	AATTGAGGTTGGACGCAACT	45.0	45.0	58.0	58.0	1326-1335 bp	1.0	82.2
rps15-ycf1_p2	AGTCGTTGACGTTTTCCCAA	ATTGAGGTTGGACGCAACTT	45.0	45.0	58.0	58.0	1325-1334 bp	1.0	82.2
rps15-ycf1_p3	AGTCGTTGACGTTTTCCCAA	TCAATTGAGGTTGGACGCAA	45.0	45.0	58.0	58.0	1328-1337 bp	1.0	82.1
rps15-ycf1_p4	AGTCGTTGACGTTTTCCCAA	TTCAATTGAGGTTGGACGCA	45.0	45.0	58.0	58.0	1329-1338 bp	1.0	82.1
rps15-ycf1_p5	AACACCATGCATTGATGGGT	ATTGAGGTTGGACGCAACTT	45.0	45.0	58.1	58.0	1593-1599 bp	1.0	81.7