Hotspot

ccsA-ndhD

SSC · 113589-113801 · 213 bp

Back to Siphocranion

Pi 0.0377

MarkerSeek score 62.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 213
variable_sites: 8
pi: 0.0377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGGTTCATTTACATTAACATCT	CCCGATTTCGTTCTTTCACT	26.1	45.0	52.1	56.1	310-311 bp	1.0	42.3
ccsA-ndhD_p2	AGTTATGGTTCATTTACATTAACA	CCCGATTTCGTTCTTTCACT	25.0	45.0	52.7	56.1	314-315 bp	1.0	42.3
ccsA-ndhD_p3	AGTTATGGTTCATTTACATTAACATCT	CCCGATTTCGTTCTTTCACT	25.9	45.0	55.7	56.1	314-315 bp	1.0	42.3
ccsA-ndhD_p4	ACATAGTTATGGTTCATTTACATT	CCCGATTTCGTTCTTTCACT	25.0	45.0	52.5	56.1	318-319 bp	1.0	42.3
ccsA-ndhD_p5	TGGTTCATTTACATTAACATCTAA	CCCGATTTCGTTCTTTCACT	25.0	45.0	52.4	56.1	309-310 bp	1.0	42.3