Hotspot

rps18-rpl20

LSC · 67778-68018 · 241 bp

Back to Siphocranion

Pi 0.0339

MarkerSeek score 73.90

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 8
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AGAACTACTGGTCCTAGAACC	TCGTAAAATACTGGCCCAAA	47.6	40.0	55.8	55.0	374-383 bp	1.0	43.6
rps18-rpl20_p2	CGACTACTAGAACTACTGGTCC	TCGTAAAATACTGGCCCAAA	50.0	40.0	56.9	55.0	382-391 bp	1.0	43.5
rps18-rpl20_p3	CGACTACTAGAACTACTGGTCCT	TCGTAAAATACTGGCCCAAA	47.8	40.0	58.2	55.0	382-391 bp	1.0	43.5
rps18-rpl20_p4	TCGACTACTAGAACTACTGGTCC	TCGTAAAATACTGGCCCAAA	47.8	40.0	58.2	55.0	383-392 bp	1.0	43.5
rps18-rpl20_p5	CGACTACTAGAACTACTGGTCC	CGTAAAATACTGGCCCAAATAGC	50.0	43.5	56.9	58.1	381-390 bp	1.0	43.5